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                            Original Research
                            
                            
                            6. 
                            
                            Amylase activity in cerical mucus and serum during 
                            estrus in normal and repeat breeder cattle -
                            
                            
                            Ashok Kumar, S. Mehrotra, S. S. Dangi, G. Singh, 
                            Mahak Singh, A. S. Mahla 
                            Vet World. 2012; 5(8): 486-488
              
               
              
              doi: 
              10.5455/vetworld.2012.
486-488
              
              
               
              
              
              
   
 
              
               
               
               
              
              
              Abstract
              
              
                            Aim: 
                            To compare the amylase activity in the cervical 
                            mucus and serum during estrus in normal and repeat 
                            breeder cattle and find out importance in fertility. 
              
                            
                            Materials and Methods: Twenty cyclic 
                            crossbred cattle were divided into two groups: 
                            normal (n =10) and repeat breeder (n =10). Animals 
                            exhibiting spontaneous estrus, were artificially 
                            inseminated (AI) twice, as per AM-PM schedule along 
                            with simultaneous collection of cervical mucus and 
                            blood. Amylase activity was measured by using kits 
                            in collected samples. 
              
                             Result: Cervical 
                            amylase activity was significantly (p<0.01) higher 
                            in normal compared to repeat breeder animals showing 
                            important role of cervical amylase in fertility 
                            without significant difference in serum profiles 
                            between these groups. 
              
                             Conclusion: Results of 
                            study suggest that cervical mucus amylase could be a 
                            facilitator of sperm transport and capacitation 
                            process in female genital tract leading to 
                            acceptable fertility in normal animals. 
              
                             Keywords: 
                            Amylase, Cervical mucus, Estrus, Repeat breeder 
                            cattle, Spermatozoa.